Figure 3
From: Calretinin Participates in Regulating Steroidogenesis by PLC-Ca2+-PKC Pathway in Leydig Cells
Expression of those factors in the PKC pathway in the Leydig cells after the overexpression and downregulation of calretinin. (A,C) The expression levels of PLC, p-PKD, PKD, p-MARCKS, MARCKS and CREB in the MLTC-1 cells infected with LV-calb2 or vector. The expression levels of PLC, p-PKD, p-MARCKS, and CREB were significantly increased in the MLTC-1 cells with over-expression of calretinin, when compared with the control group (p < 0.05 or 0.01)). The changes of total MARCKS and PKD were not significant (p > 0.05). (B and D) The expression levels of PLC, p-PKD, PKD, p-MARCKS, MARCKS and CREB in the R2C cells infected with LV-siRNA-calb2 or vector. The expression levels of PLC, p-PKD, p-MARCKS, MARCKS and CREB were significantly decreased in the R2C cells with down-expression of calretinin, when compared with the control group (p < 0.05 or 0.01)). The change of total PKD was not significant (p > 0.05). All experiments were independently repeated three times. *p < 0.05; **p < 0.01. The original blot images can be found in Supplemental Fig. S3-4.
