Figure 3
From: Cryo-EM of nucleosome core particle interactions in trans
Molecular model showing long range NCP interactions. (A) Comparison of the NCP models for cryo-EM maps A1–A5. NCP 1 is shown in blue and NCP 2 is shown in red. The two NCPs are oriented with their histone octamers facing each other, but are shifted vertically to different degrees. The dyad of both NCPs is facing in the same direction. First 37 residues of the H3-tail, not observed in X-ray structure (pdb 3lz1), are indicated as a dotted line in light blue. In most classes, the histone octamer surface remains accessible to the extrinsic factors. (B) The model showing NCP orientation in the 30 nm fiber between adjacent tetra-nucleosomal units is included for comparison. The 30 nm fiber model was built using 30 nm fiber cryo-EM map5.
