Figure 6
Fibroblasts prolong epithelial confluence and speed the onset as well as the severity of E2-induced hyperplasia. (A) Samples were stained for Hoescht (blue) and phalloidin (red) to visualize nuclei and F-actin, respectively. (B) To visualize hyperplasia, ducts were cross-sectioned and stained with Hoescht (blue). Scale bars represent 100 μm. (C) Quantification of hyperplasia. Each condition represents the cross-sections of three ducts. A two-way ANOVA was run to evaluate interactions between conditions, followed by a Tukey’s multiple comparisons test to identify significant differences. *= vs. respective vehicle (p < 0.05) and ‡= vs. respective monoculture (p < 0.05). Data is representative of three independent experiments.
