Table 3 Position and nucleotide sequences of overlapping regions between putative miR-100 target sites and transcriptional motifs on the MIR143HG promoter region.

From: MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells

Location

 

Sequence alignment

Transcriptional factors

−44

pMIR143HG

miR-100

5′-CCCGCCTCGCCCCAATACGGGGC-3′

3′-GUGUUCAAGCCUAGAUGCCCAA-5′

GR-β, Pax-5, P53 and NFI/CTF

−639

pMIR143HG

miR-100

5′-GCTGGGTCAGGATCTTCCCTGT-3′

3′-GUGUUCAAGCCUAGAUGCCCAA-5′

TFII-I, STAT4, NF-κB, RelA and RAR-α

−1439

pMIR143HG

miR-100

5′-CACAAGGCCCCGTGTGCTGCTG-3′

3′-GUGUUCAAGCCUAGAUGCCCAA-5′

GR-α, AP-2αA, Pax-5 and P53

−2112

pMIR143HG

miR-100

5′-CACAAGCCTTCCCTAACCCACC-3′

3′-GUGUUCAAGCCUAGAUGCCCAA-5′

C/EBPβ

  1. The promoter position of the nucleotide with respect to the transcription start site (defined as position 0) that is aligned to the 3′ end of each putative miR-100 binding site in MIR143HG promoter region is indicated under the “Location” column. Underlined bolded letters indicate the complementary sequences for putative binding between the microRNA seed region and transcription factor motifs on the MIR143HG promoter.