Figure 1

Specific PCR amplification of Bacillus velezensis (A) and B. subtilis subsp. subtilis (B) with the Bam249F/R and BS310F/R primer sets. (A) B. velezensis. Lane M is the size marker (1 kb DNA plus ladder; Gibco BRL). Lanes 1–16 are B. velezensis strains. Lanes 17–32 are strains of other Bacillus species, as listed in Table 2, and lane 33 is the negative control (distilled water). (B) B. subtilis subsp. subtilis. Lane M is the size marker (1 kb DNA plus ladder; Gibco BRL). Lanes 1–6 are B. subtilis strains. Lanes 7–32 are strains of other Bacillus species, as listed in Table 3 and lane 33 is the negative control (distilled water).