Table 2 Biological classification based on gene ontology annotation of proteins identified by mass spectrometry analysis of immunoaffinity-purified plasma exosomes.

From: Exosome markers associated with immune activation and oxidative stress in HIV patients on antiretroviral therapy

Biological classification

HIV-negative (n = 3)

HIV-positive (n = 4)

immune activation/inflammation

ADAM33, AZGP1, BPIFA1, BPIFB1, BTN2A2, CAMP, CD4, CSF1R, CST6, CXCL16, DDR1, DMBT1, ENO1, FERMT3, LILRB1, LILRB2, LYZ, PLTP, TFRC

ADAM33, BPIFB1, BTN2A2, CAMP, CD14, COLEC10, CPN1, CRP, CSF1R, DDR1, ENO1, FERMT3, HLA-A, HLA-B, ITGB1, ICAM2, LILRB1, PLTP, TFRC

transmembrane signaling

CD4, EFNA4, LRP8, NOTCH4, PTRF, TFRC

EFNA4, ITGA6, LRP8, NOTCH4, TFRC

extracellular vesicles/exosomes

ANXA2, APMAP, AZGP1, BPIFB1, CAMP, CAT, CD9, CD63, CD81, CDH1, ENO1, FERMT3, FLNA, GAPDH, HSPA5, LAMP1, LDHA, MYH9, PFN1, PKM, PRDX1, PRDX2, PXDN, RAP1A, SDCBP, TXN, UBA52, YWHAB, ZG16B

ADAM10, APMAP, ARF1, BPIFB1, CAMP, CDH1, CDC42, CRTAC1, ENO1, FERMT3, GAPDH, GPLD1, LAMP1, MYH9, PCYOX1, PKM, RAB1A, RAC1, RAP1A, SDCBP, SPTAN1, STOM, TLN1, UBA52

stress response

CAT, CDH1, ENO1, GAPDH, HSPA5, HSPA1L, PRDX1, PRDX2, TXN, TYMP, WARS

ADAM10, BMP1, CDH1, ENO1, GAPDH, MAP3K11, RAC1, SEPP1

oxidative stress

CAT, ENO1, GAPDH, PRDX1, PRDX2, PXDN, TPM1, TXN, WARS

CRP, ENO1, GAPDH, GPX3, ITGB1, RAC1, SLC25A33, SEPP1

fatty acid/lipid metabolism

AZGP1, CD36, CETP, DPEP3, FABP5, HGFAC, LRCOL1, PKM, PLTP

ACOX3, ADIPOQ, ANGPTL3, CD36, CETP, DPEP3, GPLD1, HADHA, HGFAC, LRCOL1, PCSK9, PKM, PLTP, SAR1A

platelets

CD36, FERMT3, ITGA2B, ITGB3, MMRN1

CD36, FERMT3, F11R, ITGA2B, ITGB3, PF4V1

  1. PANTHER and Biobase TRANSFAC tools were used for gene ontology (GO) mapping of proteins identified by mass spectrometry analysis of IAP-purified plasma exosomes from 3 HIV-negative and 4 HIV-positive subjects in the merged datasets shown in Supplemental Tables 3 and 4. GO groups were assigned to the indicated biological functions. Individual proteins may be annotated to more than one category. Proteins identified by 2 or more unique peptides are shown in bold.
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