Figure 5

Effect of hyposmotic modulation in cell fate reprogramming. (A) and (B) Reprogramming efficiency and kinetics, measured as the number of alkaline phosphatase positive colonies, with different modulation protocols in UCB CD34⁺CD133⁺ cells (normalised to the hESC medium condition). Two small molecules (VAP and BayK) with proven activity as reprogramming boosters were used for comparison with hyposmotic condition. Valproic Acid (VPA), is a chromatin modulating small molecule and BayK is a calcium agonist. (C) and (D) Fold increase in reprogramming with different hyposmotic modulation protocols in UCB CD34⁺CD133⁺ cells (normalised to the PBS condition). (E), (F) Percentage of alkaline phosphatase positive cell culture area with different hyposmotic modulation reprogramming protocols in NDHF (normalised to the PBS condition). The different osmotic modulation protocols are described in the legend of each graph. The variations presented are the mean value ± SEM (n ≥ 3) and the changes are statistically significant at the time points highlighted in the graphs (*p value < 0.05; **p value < 0.01).