Figure 6

Effect of hyposmotic modulation in cell fate transdifferentiation. (A,B) CD11b expression by the HAFTL C10 cell line during the transdifferentiation. Although the images provided are from a single experiment, the same experiment was done at least two more times with similar results. (C) CD11b expression (% of cells expressing CD11b relative to PBS-control) by the HAFTL C10 cell line at day 4 of transdifferentiation. (D) CD11b expression (Geo MFI, geometric mean fluorescence intensity) by the HAFTL C10 cell line at day 4 of transdifferentiation. (E) Transcriptional characterization of CEBP-family genes assessed at day 4 of transdifferentiation in HAFTL C10 cell line by qRT-PCR (ratio between the expression levels (2−ΔΔCT) in the two transdifferentiation conditions) (ANOVA test used). (F) Gene expression of CEBP-related transcription factors assessed at day 4 of transdifferentiation in HAFTL C10 cell line by qRT-PCR (ratio between the expression levels (2−ΔΔCT) in the two transdifferentiation conditions) (ANOVA test used). The different osmotic modulation protocols are described in the legend of each graph. The data presented corresponds to the mean value ± SEM (n ≥ 3) and the changes are statistically significant at the time points highlighted in the graphs (*p value < 0.05).