Figure 5

Amiloride blocks touch-induced calcium responses in RnB neurons. (a,b) Averaged calcium responses (a) and maximum ΔF/F0 changes (b) in R1B-R3B neurons induced by mechanical stimulation of 15 μm displacement at the position of rays 1–3 in wild type worms with bath solution, GdCl3, amiloride or after rinsed amiloride out with bath solution. Solid lines show the average fluorescence changes and the shading indicates SEM. n ≥ 7. Data are mean SEM. ***P < 0.001, unpaired Student’s t-test.