Figure 9
Systemic inhibition of CFD and deletion of C4 protects POS from laser-induced BRB disruption. (a) C1q immunoreactivity at the interface of blue opsin-positive cone outer segments and the apical border of the RPE, 24 hours after laser injury. Images are taken 300 μm nasally of the laser injury. (b) Cone outer segment length in naïve (C4+/+ no laser), C4+/+ mice treated with isotype Ab (Ctrl) and C4−/− mice treated with a-fD, 72 hours after BRB disruption with a laser. ****p < 0.0001, 1-way ANOVA with Tukey’s multiple comparisons test. (c) H&E stained section of a lesion site 24 hours after laser injury (white arrows) indicating POS length (close-up of boxed inset, lower panels) in C4−/− mice treated with a-fD compared to C4+/+ mice treated with control Ab. The POS layer is located between the dashed lines. (d) POS length measured as a function of distance from the lesion site or, in the naïve group, the distance from a site that corresponds anatomically to the site of the lesion in the treated group. Scale bar = 25 μm. *p < 0.05 Mann-Whitney test vs C4−/− a-fD treatment group, n = 5. ONL = outer nuclear layer; POS = photoreceptor outer segment.
