Figure 4

Changes in cell growth, migration, and the actin cytoskeleton. (a,b) Scratch-wound analysis for 2D migration over 48 hours. t-test at 48 hours 8MBGA p = 0.04. (c,d) Trypan blue dye exclusion assay to measure cell growth over 72 hours; 42MBGA p = 0.0066. (e) Mean thickness of F-actin filaments assessed by FIJI plug-ins as denoted in Methods section. Mann-Whitney U test 8MBGA p = 0.001. (f) Representative image of quantification in e.