Figure 4 | Scientific Reports

Figure 4

From: A targeted boost-and-sort immunization strategy using Escherichia coli BamA identifies rare growth inhibitory antibodies

Figure 4

Sorting hybridomas with amphipol- BamA followed by high-throughput hybridoma production yields large panel of purified BamA+ mAbs. (a) Representative yields (~100 μg on average; grey bars) and final purified concentrations (~150 μg/mL on average; black bars) from one plate of purified hybridomas. (b) Panel of 1632 BamA-sorted purified hybridomas is highly enriched for BamA binding by ELISA (~88.5%), ΔwaaD FACS (53%), or even K12 FACS (13.5%).

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