Figure 5

3D-bioprinted construct. (a) Microscope image of the 3D-bioprinted tissue construct immediately after printing on day 1. (b) Microscope image of the 3D-bioprinted tissue construct on the Kenzan micro-needles after a week of continuous pulsatile perfusion. (c) Bright field image of the same construct after its removal from the Kenzan. (d) Photograph of the perfused 3D-bioprinted construct after a week of continuous pulsatile perfusion. (e) Histology of the scaffold-free 3D-bioprinted liver tissue which was kept statically in the culture media for one week. Solid bar indicates 100 µm. (f) Histology of the scaffold-free 3D-bioprinted liver tissue which was continuously perfused using the FABRICA for one week, showing improved survival of cells in the tissue. Solid bar indicates 100 µm. (g) Histology of the scaffold-free 3D-bioprinted liver tissue which was kept statically in the culture media for one week. Solid bar indicates 50 µm. (h) Histology of the scaffold-free 3D-bioprinted liver tissue which was continuously perfused using the FABRICA for one week, showing improved survival of cells in the tissue. Solid bar indicates 50 µm.