Table 1 Medium formulations and flow rates.
From: FABRICA: A Bioreactor Platform for Printing, Perfusing, Observing, & Stimulating 3D Tissues
Medium Formulations | Pump Flow Rates |
|---|---|
Sterile Water + 0.5 mg/mL cornstarch | 1 mL/minute |
Liver Construct Culture Medium ([LCCM], (αMEM/EGM-MV, 3:1) αMEM (Invitrogen) + EGM-MV (Lonza) + HEPE Buffer + 1% antibiotic + 10% horse serum + 10% FBS + 0.5 mg/mL cornstarch | 3 mL/minute |
Dulbecco’s Modified Eagle Medium + 0.5 mg/mL cornstarch | 5 mL/minute |
Porcine Blood (Pig blood + 1 U/mL heparin + 1% antibiotic) | 7 mL/minute |
Human Blood (Human blood + 1 U/mL heparin + 1% antibiotic) | 10 mL/minute |
