Figure 2 | Scientific Reports

Figure 2

From: Identification of a unique Radical SAM methyltransferase required for the sp3-C-methylation of an arginine residue of methyl-coenzyme M reductase

Figure 2The alternative text for this image may have been generated using AI.

PCR based illustration of the M. acetivorans WWM1 and Mko4551 genotypes. (a) Verification of genotypes by PCR. Amplification of the ma4551 region using primers DD15 and DD73 from wild type DNA yielded a 2.2 kb fragment (lane 1), which was not obtained from Mko4551 DNA (lane 2). A part of the pac-hpt cassette was successfully amplified from Mko4551 DNA using primers Ma5 and Ma6 (lane 4) (ca. 2 kb), but not from wild type DNA (lane 3). Lane L: Quick-Load® 1 kb DNA Ladder (New England BioLabs). (b) Genotype of the M. acetivorans WWM1 and Mko4551 with the primer pairs used for PCR, their binding sites (purple) and the sequenced products (turquoise arrows). Pac-puromycin transacetylase gene and hpt-8-aza-2,6-diaminopurine hypoxanthine phosphoribosyl transferase resistance gene flanked by two Flp recombinase recognition sites (FRT).

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