Figure 5
From: The Protease Activated Receptor2 Promotes Rab5a Mediated Generation of Pro-metastatic Microvesicles
Trypsin Dependent Rab5a Activation Promotes Migration and Invasion of MDA-MB-231 Cells. Cells were transfected with Rab5a WT, Rab5a DN and Rab5a CP and incubated for 48 hours at the end of which serum starvation was given followed by trypsin addition. Migration potential of the cells were analyzed by wound healing assay as described briefly in methods and accordingly quantification was made by GraphPad Prism5 (A) and (B). Cells were treated with various Rab5a constructs and placed on top of a transwell membrane previously coated with matrigel in serum free media. Cells were treated with trypsin and the lower compartment is filled with complete media. Cells that were invaded through the membrane were fixed and stained with crystal violet solution. Images were taken and number of invaded cells was counted and quantified accordingly (C) and (D). Data presented over here are as Mean +/− S.E. of the Mean after performing the experiments at least thrice and differences are statistically significant at p < 0.05 using student’s t-test and ‘ns’ represents non-significant differences.
