Figure 2
From: Identification of new EphA4 inhibitors by virtual screening of FDA-approved drugs
Nilotinib inhibits ephrin-A and EphA4 interaction, and EphA4-dependent signaling and cellular functions. (a) Dose–response curve of the inhibitory activity of nilotinib against the interaction of mouse EphA4 with ephrin-A1. (b) Nilotinib attenuated the ephrin-A1 (A1)-induced EphA4 tyrosine phosphorylation in rat hippocampal neurons. Lysate was immunoprecipitated with EphA4 antibody and subjected to western blot analysis for P-Tyr. (c) Nilotinib inhibited ephrin-A1–stimulated growth cone collapse in cultured hippocampal neurons (mean ± SEM, ≥75 neurons for each group from 3 experiments). ***p < 0.001; two-way ANOVA followed by the Bonferroni post hoc test. Cont (ephrin-A1–treated neurons alone).
