Figure 6
From: High-speed Fourier ptychographic microscopy based on programmable annular illuminations
Video-rate phase imaging of HeLa cells in vitro using AIFPM with 4 raw images and an acquisition time of 0.04 s per frame. (A) One frame of the full FOV phase reconstruction using a 10× objective and achieving 0.8 NA resolution. (B,C) Enlarged images of the red-boxed and blue-boxed regions in (A). (D1,D2) Sample frames of reconstructed video (see Supplementary Video 2) for zoom-in view of two selected regions respectively at the maximum frame rate of 25 Hz within 1 s or at about 1.47 min intervals across 7.33 min. (E) Line profiles for tracking subcellular features’ sub-pixel movement within 1.8 s.
