Figure 2 | Scientific Reports

Figure 2

From: Possible role of the Nipah virus V protein in the regulation of the interferon beta induction by interacting with UBX domain-containing protein1

Figure 2

Identification of the binding domains of NiV V and UBXN1. (A,B) Schematic diagrams of the C-terminal domain of V protein (A) and its deletion mutants (B) are shown. (C) Wild-type NiV V and deletion mutants of NiV V were expressed in HEK293T cells, and an immunoprecipitation assay and western blotting were performed as described in Fig. 1B. (D) A schematic diagram of GST-tagged deletion mutants of UBXN1 is shown. (E) The interactions between GST-tagged UBXN1 mutants and myc-tagged NiV V were evaluated with a GST pull-down assay. Pulled-down proteins were detected with western blotting. The blots presented in (C,E) were cropped from different images to improve clarity. Full-length blots are presented in Supplementary Figure S1.

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