Figure 2 | Scientific Reports

Figure 2

From: Informative three-dimensional survey of cell/tissue architectures in thick paraffin sections by simple low-vacuum scanning electron microscopy

Figure 2

Preliminary examinations to determine the deal thickness of paraffin sections. (a,b) Lung. Montage image of whole section (a) and high-power view of the pulmonary alveoli with its wall face image (b). (c–f) Correlative light (H&E staining) and Thick PS-LvSEM microscopy of rat lung paraffin sections in a series of 5, 15, 30, and 50 µm thickness. The sectioned features of alveolar septum are dominant in the 5 µm (c) and 15 µm (d) sections. On the other hand, the wall-face features occupy more than half the area in the 30 µm (e) and 50 µm (f) sections. Note the unavoidable cracks in the 50 µm sections. (g–i) Comparison of oblique-section images of pulmonary pleura in 5 µm (g), 15 µm (h), and 30 µm (i) sections.

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