Figure 3 | Scientific Reports

Figure 3

From: Informative three-dimensional survey of cell/tissue architectures in thick paraffin sections by simple low-vacuum scanning electron microscopy

Figure 3

Preliminary examinations to determine the stainability of 30 µm paraffin sections. (a–e,g,h) Rat auricle and (f) larynx. (a) No stain. (b) 1% uranyl acetate in 70% methanol (UA) alone. (c) Reynolds’ lead citrate solution (LC) alone. (d) UA and LC. (e,f) High-power view of elastic cartilage (e), and hyaline cartilage (f) in larynx stained with LC alone. Note the remarkable LC-stainability of the cartilage, as shown in the high-power view of hyaline matrix (right in f). (g) Platinum coating. (h) Comparison between UA/LC staining (upper) and platinum coating (lower) on skeletal muscle fibre. The skeletal muscle striation is significantly masked by the platinum coating.

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