Figure 8 | Scientific Reports

Figure 8

From: Informative three-dimensional survey of cell/tissue architectures in thick paraffin sections by simple low-vacuum scanning electron microscopy

Figure 8

Application of Thick PS-LvSEM to oncological experiments using SUIT-58 cell line. (a) Illustration of the comparative experiments between xenograft and three-dimensionally cultured cells. (b,c) Light micrographs of H&E stained 5 µm sections and (d–g) Thick PS-LvSEM micrographs of 30 µm sections. Note the similar profiles of vacuole formation enclosing necrotic cell debris (arrowheads). (f) Illustration of the four-segmented BSE detectors (left) and collected micrographs by each detector (right). (g) Topographic images reconstructed from the raw micrographs of the four-segmented BSE images.

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