Figure 4
From: A direct tissue-grafting approach to increasing endogenous brown fat
Single-step browning of human WAT tissue ex vivo. Experimental design was shown in Fig. 1. (a) Human WAT fragments cultured in browning media (top panel) and control media (middle panel) for 3 weeks and native human WAT (not cultured, bottom panel). Tissue was stained for UCP1 expression (red) and counterstained with LipidTox (green) and Sytox nuclear stain (blue). Scalebars are 50 µm. (b) RNA expression levels in human fragments cultured in control versus browning media. Left to right: UCP1, PRDM16, DIO and LEPTIN. (c) Western blot analysis of UCP1 protein expression in human WAT fragments cultured in control (left) or browning (right) media for 2 weeks. Protein expression is normalized to GAPDH. Compared to control tissues, UCP1 protein expression in tissues cultured in browning media was significantly greater (p < 0.0001). (d) Citrate synthase analysis of UCP1 activity in control media and browning media. Student’s t-test performed (p < 0.0001).
