Figure 6
Circulating erythrocytes express genes of a large number of secretory molecules. (a) Relative gene expression levels of secretory molecules (growth factors and MMPs) as well as Newtic1 in whole blood. We carried out mRNA-seq (n = 3) and aligned the sequence reads on TOTAL (Supplementary Table S3). (b) PCR confirmation of gene expression in PcNobs (n = 3). PcNobs were purified (99.3–99.8%) from whole blood through a sieve and isopycnic centrifugation (See Methods and Supplementary Figure S9). The representative data set shown here was obtained with the same cDNA sample (+) and its original RNA sample without reverse transcription (−). PCR products (+ and −) of growth factors, those of MMPs and Newtic1, and that of nsCCN were electrophoresized on the same gel each. We detected the transcription of all of the genes listed in (a) (asterisk). As for nsCCN, two bands corresponding to the variants were detected. M: marker.
