Figure 1

Nde1 is expressed in oligodendrocyte lineage cells in the mouse carpus callosum. (a) In situ hybridization analyses of brain sections from P1 (left panel), P14 (middle panel) and adult mice (right panel) with antisense RNA probes to Nde1 mRNA. (b–g) Double in situ hybridization–immunohistochemistry analyses of brain sections from P14 mice with antisense RNA probes for Nde1 mRNA and antibodies against Olig2 (b,e), CC1 (c,f), and PDGFRα (d,g) at low magnification (b–d) and high magnification (e–g). Arrowheads indicate colocalization. (h) Western blot analysis of NDE1 and MBP protein expression in the corpus callosum at different stages. (i,j) Densitometric quantification of three independent western blot analyses. *P < 0.05; **P < 0.01 versus P14 by Tukey-Kramer post-test following one-way ANOVA (n = 3). Scale bars: (a) P1: 0.2 mm, P14 adult: 0.2 mm, (b–g) 20 µm. Full-length blots are shown in Supplementary Fig. S4a.