Figure 6 | Scientific Reports

Figure 6

From: PLGA nanoparticles co-delivering MDR1 and BCL2 siRNA for overcoming resistance of paclitaxel and cisplatin in recurrent or advanced ovarian cancer

Figure 6

Induction of apoptotic and necrotic cell death on MDR ovarian cancer cells by combination treatment. MDR SKOV3-TR cells were treated sequentially with PLGA NPs containing MDR1 and BCL2 siRNA (37.5 nM, respectively) and paclitaxel (5 μM), as represented in a schematic diagram. Based on flow cytometry, induction of apoptosis and necrosis was investigated in triplicate, and a representative experiment set is demonstrated here. Each percentage of fully apoptotic, early apoptotic, and necrotic cells were indicated in the corresponding quadrant, and also plotted below. Both the fully and early apoptotic cells are included in the percentage of apoptosis, and the results are shown as the mean ± s.d. (n = 3).

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