Figure 1

Climbing Response During Lifespan. The climbing index was assessed as the time taken for the first fly to climb 17.5 cm. (A) Expression of hKv9.2 and hKv9.2-D379E channels throughout neuronal development and effects on climbing response during lifespan. The mean climbing index ± SEM as a function of age is shown for Elav/+, Elav > hKv9.2 and Elav > hKv9.2-D379E. Each point represents the mean of 10 flies except for: Week 1, Elav > hKv9.2 (n = 9); Weeks 1–8, Elav > hKv9.2-D379E (n = 11); Week 6, Elav/+ (n = 7); Week 8, Elav/+ (n = 1). Flies expressing either Elav > hKv9.2 and Elav > hKv9.2-D379E displayed significantly faster climbing (p < 0.0003) throughout lifespan. (B) Expression of hKv9.2 and hKv9.2-D379E channels post developmentally in neurons and effects on climbing response during lifespan. The mean climbing index ± SEM as a function of age is shown for Elav-gal4/+ Gal80^ts, Elav-gal4 > Gal80^ts hKv9.2 and Elav-gal4 > Gal80^ts hKv9.2-D379E. Each point represents the mean of 10 or 9 flies. Flies expressing either Elav > hKv9.2 and Elav > hKv9.2-D379E Elav-gal4 > Gal80^ts displayed significantly faster climbing (p = 0.04–0.0001; weeks 3–5) during lifespan.