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Figure 2

From: In Utero and Postnatal Exposure to High Fat, High Sucrose Diet Suppressed Testis Apoptosis and Reduced Sperm Count

Figure 2

Cross sections of normal (animal #198) and azoospermic (animal #199) testes (A,B) and epididymides (C,D). (E) Immunostaining of SOX9 (Sertoli cell marker), DNA counterstaining by DAPI, and TUNEL labeling of apoptotic germ cells in the seminiferous tubules of male #199. The spacing of Sertoli cells (arrowheads) along tubule basement membrane appears normal, and the TUNEL positive cells are spermatogonia and spermatocytes, as in other males. Mice #198 and #199 were brothers exposed to HFHS in utero. After weaning, mouse 198 was on CD and 199 on HFHS diet.

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