Figure 5 | Scientific Reports

Figure 5

From: In Utero and Postnatal Exposure to High Fat, High Sucrose Diet Suppressed Testis Apoptosis and Reduced Sperm Count

Figure 5

Representative images of TUNEL assay on seminiferous tubule cross-sections from four animals per treatment (A: CD-CD, B: CD-HFHS, C: HFHS-CD, and D: HFHS-HFHS diet group). (E) Identification of Sertoli cells by anti-SOX9 antibody in red, a Sertoli cell-specific marker (indicated by arrows in E’). SOX9 labeling does not overlap with green fluorescence of TUNEL probe, indicating germ cells, rather than Sertoli cells are apoptotic in the examined tissues. (F) Identification of spermatogonia by anti-UCHL1 antibody in red, a specific spermatogonial marker (arrows). Inset F’ shows the blue and red channel detail of two large, green-labeled TUNEL-positive cells visible in panel F. Colocalization of TUNEL labeling and UCHL1 (F) indicates that apoptosis occurs in the spermatogonia, close to basement membranes (see also white arrows in panel A’).

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