Table 1 Some key DAP spots in Bd21 seedling roots with significant accumulation changes under three stress treatments.

From: Identification of differentially accumulated proteins involved in regulating independent and combined osmosis and cadmium stress response in Brachypodium seedling roots

Spot no.a

Protein name

Accession no.b

Protein Scorec

Protein Score C. I.%d

Predicted subcellular localisatione

Average %vol ratiof

PEG

Cd

PEG + Cd

1 day

3 day

2 day

4 day

1 day

3 day

2 day

4 day

1 day

3 day

2 day

4 day

Signal transduction:

60

Guanine nucleotide-binding protein subunit beta-like protein A (receptor for activated C kinase 1A, RACK1A)

XP_003567944.1

640

100

Cyto

0.9

1.6

0.6

1.3

0.8

1.4

1.8

0.7

0.7

0.7

1.3

0.4

74

14–3–3-like protein GF14-D

XP_003577480.1

127

100

Cyto

0.6

0.6

0.4

0.7

0.5

0.5

0.6

0.4

0.7

0.8

0.4

0.5

184

Nucleoside diphosphate kinase II NDPK2

AGK91194.1

545

100

Cyto

0.5

0.6

0.4

0.4

0.7

0.8

0.7

0.6

0.6

0.5

0.6

0.7

201

14-3-3-like protein A

XP_003560774.1

229

100

Plastid

0.7

0.7

0.8

1.1

1.0

0.7

0.6

1.3

0.4

0.5

1.1

0.6

206

14–3–3-like protein GF14-B

XP_003579911.1

487

100

Cyto

1.1

1.1

0.8

1.7

0.4

1.6

0.9

1.2

1.3

1.9

0.8

0.0

Energy metabolism Energy metabolism

31

Triosephosphate isomerase

XP_003568647.1

665

100

Cyto

1.6

1.4

0.6

1.3

1.2

1.9

1.2

1.4

1.2

0.9

1.4

1.6

33

Triosephosphate isomerase

XP_003568647.1

522

100

Cyto

1.5

1.1

0.8

1.1

1.3

1.3

0.7

1.2

0.8

1.3

1.3

1.5

45

Probable ATP synthase 24 kDa subunit

XP_003574053.1

199

100

Mito

1.2

1.6

1.6

1.4

0.9

1.6

1.3

1.3

2.9

1.6

1.5

2.5

72

Glyceraldehyde-3-phosphate dehydrogenase, GAPDH

XP_003573324.1

429

100

Cyto

1.4

0.9

0.7

1.9

2.3

2.5

1.0

1.3

2.4

1.3

1.7

0.3

92

Malate dehydrogenase, MDH

XP_003574062.1

901

100

Cyto

0.8

0.7

0.5

0.6

0.9

0.7

0.9

1.1

0.8

1.0

0.8

1.2

152

2,3-bisphosphoglycerate-independent phosphoglyceratemutase-like PGM

XP_003564482.1

681

100

Cyto

1.6

1.8

1.0

1.1

1.3

1.7

0.8

0.7

1.0

1.1

1.0

0.7

331

Probable ATP synthase 24 kDa subunit

XP_003574053.1

316

100

Mito

2.6

0.9

0

1.2

0.7

0

3.6

0.7

0

0

1.8

0

526

ATP synthase subunit beta

XP_003569580.1

578

100

Mito

1.2

0.3

0.6

0.8

1.5

0.8

1.4

1.5

2.3

2.1

0.9

1.2

555

Enolase

XP_003573806.1

418

100

Cyto

3.4

2.9

3.2

5.4

3.8

3.2

1.2

1.6

1.6

2.8

2.8

5.9

Protein metabolism

18

40 S ribosomal protein S7

XP_003558127.1

151

100

Cyto

1.2

1.4

1.6

1.3

1.1

1.4

1.0

0.8

1.0

0.9

1.7

2.9

159

Heat shock 70 kDaprotein,mitochondrial-like

XP_003570534.1

559

100

Cyto

1.7

1.3

1.6

1.3

0.8

1.6

0.3

1.5

0.8

0.7

1.1

0.5

165

Heat shock cognate 70 kDa protein 2-like

XP_003578898.1

233

100

Cyto

1.3

0.3

0.3

0.3

1.9

1.2

0.4

1.7

0.4

0.3

0.3

0.7

166

Heat shock cognate 70 kDa protein 2-like

XP_003578898.1

441

99.678

Cyto

1.2

0.7

0.9

1.4

1.5

1.1

0.1

0.7

1.4

1.9

1.2

0.9

264

Protein disulfide-isomerase-like isoform 2

XP_003577737.1

598

99.998

ER

1.1

0.9

0.4

0.6

1.0

2.1

0.6

0.5

0.7

0.7

1.6

2.1

272

Heat shock 70 kDa protein, mitochondrial-like

XP_003570534.1

368

100

Cyto

0.7

0.6

0.8

0.5

1.5

0.6

0.9

0.9

0.4

0.4

0.6

0.4

354

Denticleless protein homolog A-like

XP_003559543.1

67

100

Nucl

0.7

0.3

0.7

1.5

0.5

0.8

0.5

0.9

0.5

0.0

0.4

0.0

492

Protease 2-like

XP_003560292.1

408

100

Cyto

0.5

0.3

0.5

0.6

1.0

0.7

0.0

0.3

0.4

1.2

0.5

1.2

Detoxification and stress fense

7

Superoxide dismutase [Cu-Zn]

XP_003574947.1

389

99.998

Cyto

0.6

0.7

0.4

0.9

0.7

0.9

0.7

0.6

1.2

0.8

0.9

1.1

8

Superoxide dismutase [Cu-Zn] 4A-like

XP_003574947.1

71

100

Cyto

0.8

0.8

0.3

0.8

0.9

1.1

0.7

0.9

0.9

0.9

1.0

1.0

21

2-Cys peroxiredoxin BAS1

XP_003575085.1

327

100

Cyto

0.8

0.7

0.0

0.9

1.2

0.9

1.0

0.7

0.8

1.1

1.0

0.8

22

2-Cys peroxiredoxin BAS1

XP_003575085.1

350

100

Plastid

1.0

1.0

1.7

1.4

0.9

1.0

1.1

0.9

1.8

1.1

0.9

0.7

25

probable glutathione S-transferase GSTU6-like isoform 3

XP_003580395.1

125

100

Cyto

0.4

0.6

1.6

1.0

0.7

1.0

0.8

0.4

1.0

0.9

0.9

0.9

27

glutathione S-transferase DHAR2-like isoform 1

XP_003569014.1

147

100

Cyto

0.8

1.0

0.9

0.8

0.8

1.3

1.1

1.0

0.8

1.1

1.1

1.2

30

glutathione S-transferase DHAR2-like isoform 1

XP_003569014.1

245

100

Cyto

0.6

1.2

0.0

2.8

0.0

3.9

1.6

1.0

2.2

2.0

2.3

2.7

34

Glutathione S-transferase 1-like

XP_003569881.1

532

100

Cyto

2.3

2.6

1.6

3.5

1.7

1.6

1.4

1.9

2.6

3.1

1.5

2.0

37

L-ascorbate peroxidase 2, cytosolic-like

XP_003562395.1

90

100

Cyto

0.9

1.1

0.7

1.0

1.0

1.4

0.7

0.9

1.3

0.9

0.8

0.7

38

L-ascorbate peroxidase 1, cytosolic-like

XP_003558178.1

574

100

Cyto

1.3

1.3

1.9

1.1

1.1

1.2

1.1

1.0

1.1

2.5

1.8

1.1

39

L-ascorbate peroxidase 1, cytosolic-like

XP_003558178.1

109

100

Cyto

0.7

1.2

0.8

0.6

0.7

0.9

0.9

0.7

0.6

1.0

0.7

1.0

273

probable nucleoredoxin 1-1-like

XP_003562828.1

150

100

Cyto

1.0

0.6

0.4

0.6

0.9

1.3

0.4

0.8

1.4

1.6

1.4

0.9

398

delta-1-pyrroline-5-carboxylate dehydrogenase 12A1, mitochondrial-like

XP_003568012.1

285

100

Mito

0.0

1.7

0.8

2.0

0.8

1.6

1.6

1.0

0.0

1.2

2.5

0.3

588

L-ascorbate peroxidase 1

XP_003558178.1

583

100

Cyto

1.4

2.1

1.2

2.4

0.8

0.8

1.5

1.3

1.0

1.6

0.9

0.6

  1. aSpot number as given in Fig. 4.
  2. bAccession number: according to the NCBI database.
  3. cProtein Score: statistical probability of true positive identification of the predicted protein calculated by MASCOT with 0.3 peptide tolerance and one allowed missed cleavage.
  4. dProtein Score C.I.%: the PMF score percentage of protein sequence (Confidence interval: Protein Score C.I.% ≥ 95%).
  5. eCyto, cytoplasm; plastid; Mito: mitochondria; Nucl: nuclear; ER: endoplasmic reticulum.
  6. fAverage %vol ratio: the average %vol of control is set to 1.
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