Figure 5
Altered media change protocol for the gut MPS maintains glucose and lactate at steady state levels. Experimental validation of the computationally developed partial media change protocol for the gut MPS is illustrated. (a) Predicted quasi-steady state operation maintaining physiological glucose levels in both apical (solid lines) and basal (dashed lines) compartments align well with measured glucose (black symbols) and lactate (red symbols) levels in both compartments (apical: circles; basal: squares). (b) Assessment of TEER values showed an intact and comparable barrier function over this extended time-course to the complete 2-day media change. Glucose and lactate concentrations were determined immediately before media replenishment and glucose supplementation.
