Figure 7
Simulation of quasi-steady state operational media change protocols for an integrated 3-MPS platform. Operational strategies were investigated for an integrated 3-MPS platform (a) consisting of gut, liver and cardiac MPSs as well as a mixing chamber to identify protocols that could maintain glucose and lactate concentration within desired ranges. (b) Desired glucose concentration: 4–7 mM (between red planes); (c) desired lactate concentration: <20 mM (below red plane). Media change protocols simulated were replacement of media volume every 24 h from the basal site of the gut (0-100%, 10% steps) and supplementation of glucose every 24 h to the apical site of the gut (0–10 mM, 1 mM steps) for 10 days, with the final glucose (d) and lactate (e) concentrations represented by the simulated surfaces. (d) Desired glucose: 4–7 mM (between red planes); (e) desired lactate: below 2 mM (physiological levels; below red plane). The range of media change protocols were simulated as in (b) and (c) except the replacement/replenishment interval was 6 h. The simulated surfaces are the final glucose (left) and lactate (right) concentrations after 10 days. The color of the surfaces in (b), (c), (d), and (e) corresponds to the colorbar on the right-hand side and to values on the z-axis, i.e. represents glucose (left, b and d) and lactate (right, c and e) concentrations.
