Table 1 Overview of MPS configuration, MPS-specific hardware and cell parameters.
MPS | Cardiac | Liver | Gut |
|---|---|---|---|
Cell type | iPSC-derived cardiomyocytes | Human primary hepatocytes | Cancer cell line (differentiated Caco-2/HT-29) |
Format | 96-well | LiverChip | 12-well transwell |
Maturation/Differentiation duration post seeding (days) | 2 | 3 | 21 |
Seeding density | 0.3 × 106/cm2 | 6 × 105/scaffold | 0.9 × 105/cm2 (9:1 ratio) |
Operating media volume (ml) | 0.2 | 2.1 | Apical: 0.5 Basal: 1.5 |
Media volume per seeded cell (nl/cell) | 2.1 | 3.5 | 17 |
Media flow | static | recirculation | static |
Media change frequency | 2 days | 2 days | 2 days |
