Figure 2
NOS activity. (a) L-citrulline content in hPMEC from pregnancies during which the mother exhibited MPH or MSPH. Cells were pre-incubated with L-arginine (100 μmol/L, 30 min) in the absence (white) or presence (black) of 100 μmol/L NG-nitro-L-arginine methyl ester (L-NAME, 30 min). (b) L-NAME inhibited L-citrulline content (from the data in a) in hPMEC from MPH (white) and MSPH (black) placentas. (c) Representative western blot for total eNOS expression in MPH or MSPH cells (ß-actin: internal control). Lateral panel: eNOS/ß-actin densitometry ratios normalized to 1 in the MPH group. In (a), *P < 0.05 versus values in the absence of L-NAME, †P < 0.05 versus MPH values. In (b), *P < 0.05 versus MPH values. Values are presented as the mean ± S.E.M. (n = 6).
