Figure 3

Raman spectroscopy of round and irregular otoliths. Raman spectroscopy profiles for aragonitic round otoliths (A) and calcitic irregular otoliths (B) in gilthead sea bream larvae exposed to pH7.3. Typical calcium carbonate ν1 and ν4 peaks are indicated and the shift position of each peak is indicated in brackets. Representative round and irregular otoliths are shown for each spectrum. Spectrums are showed separately in order to facilitate the visualization of the different peaks.