Figure 3
Induction of the genes fet3a, fet3b and fet3c by low iron availability. Upper panels show levels of fet3a, fet3b and fet3c mRNAs (A,B and C, respectively). Total RNA was extracted from mycelia of the wild type strain R7B grown in liquid culture for 24 hours in YNB pH 3.2, then the mycelia was washed and grown in either L15 or L15 + FeCl3 350 μM. To analyze expression of the fet3 genes in response to iron limitation, the mycelia grown in L15 were washed and transferred to an iron depleted media (with FBS or 1,10-phenanthroline) and samples were taken at 30 min, 60 min and 120 min. Middle panels show mRNA loading controls, which were performed by re-probing the membranes with a rRNA 18S probe. Lower panels show relative accumulation of fet3a, fet3b and fet3c mRNAs (A, B and C, respectively) by normalization with the 18S rRNA signals. Significant differences respect to control L15 medium are indicated with an *(p < 0.01, unpaired t-test). The cropped blots are displayed in the main figure, the black lines surrounding blots indicate the cropping lines. The scanned full blots are presented in Supplementary Fig. 2.
