Figure 7
Differential expression of the genes fet3a, fet3b and fet3c during dimorphic development. Upper panels show levels of fet3a, fet3b and fet3c mRNAs (A,B and C, respectively) during yeast and mycelial growth. Two cultures of 50 ml of YPG 4.5 inoculated with 106 spores/ml were grown in a tightly closed 50-ml conical tube for 15 hours to induce yeast growth. Next, one of the tubes was opened and transferred to an open 500 ml flask in which it was shaken for 2 hours to promote the mycelial growth. These two cultures were repeated adding either 1,10-phenanthroline 10 μM or FeCl3 350 μM. Middle panels show mRNA loading controls, which were performed by re-probing the membranes with a rRNA 18S probe. Lower panels show relative expression of the genes fet3a, fet3b and fet3c mRNAs (A,B and C, respectively) by normalization with the 18S rRNA signals. The cropped blots are displayed in the main figure, the black lines surrounding blots indicate the cropping lines. The scanned full blots are presented in Supplementary Fig. 4.
