Figure 5
Quantitative analysis of the matching quality between histological data and microCT data for both, the laser and the microtome cut tissue sections: (A) and (B) show the results of the block matching analysis between the PHR CT data of (A) the paraffin embedded and (B) the resin embedded mouse lung with their corresponding histological images. The local displacement vectors are depicted in red, while the magnitude of mutual information (MI) is illustrated in size and in colour (from red to green) of the circles. A higher value of MI indicates a closer match between the structures in CT and histology. Areas with less than 1% of brightness were not processed. In (B), the resin embedded tissue, nearly no local displacement (despite some areas dominated by noise from the embedding material in the CT scan) and a higher degree of local MI are found compared to (A) the paraffin embedded lung. The overall displacement index DI is a lot lower for the laser cut resin sample (DI = 5.7 ± 0.9; n = 4) than for the paraffin sample cut with the standard microtome (DI = 10.2 ± 0.5, n = 4).
