Figure 7
Real-time reverse-transcription PCR revealed altered expression of genes related to sarcomere assembly and heart contractility in embryonic hearts from Tg (myl7: EGFP; myl7: H2AFZ mcherry) and VCAP1X2 mutant at 96 hpf. (A) Dilated cardiomyopathy-related genes encoding sarcomeric proteins, including myh6, myh7, vmhcl, tnnt2a, mypn and fhl2a, were significantly decreased, while expression of two natriuretic peptides (nppa and nppb) were substantially increased. (B) smyd1b was significantly reduced in VCAP1X2 mutant accompanied by an induction of expression of hsp90aa1.1 and cryabb. (C) Ca2+ handling regulators (ryr2b and slc8a1a) were significantly downregulated in VCAP1X2 mutants. (D) Genes related to epicardium formation were not significantly altered between WT and VCAP1X2 mutants. Error bars indicate standard deviation. Student’s t-test, *p < 0.05. ***p < 0.001.
