Figure 1
From: PCR-Stop analysis as a new tool for qPCR assay validation
The real amplification curve of a qPCR assay. The range of observable and significant increase in fluorescence based on amplification of the PCR product is small. The exponential phase is mostly masked by the background fluorescence of the uncoupled probe dye. Therefore PCR-Stop analysis is an indirect approach depicting events during the first amplification cycles of qPCR.
