Table 2 Pre-runs.
From: PCR-Stop analysis as a new tool for qPCR assay validation
Initial denaturation | Cycle | Cycle number | ||
|---|---|---|---|---|
prfA | 0 | — | — | — |
1 | 94 °C, 2 min | 94 °C, 15 s–64 °C 1 min | 1 | |
2 | 94 °C, 2 min | 94 °C, 15 s–64 °C 1 min | 2 | |
3 | 94 °C, 2 min | 94 °C, 15 s - 64 °C 1 min | 3 | |
4 | 94 °C, 2 min | 94 °C, 15 s–64 °C 1 min | 4 | |
5 | 94 °C, 2 min | 94 °C, 15 s–64 °C 1 min | 5 | |
exB | 0 | — | — | — |
1 | 95 °C, 5 min | 95 °C, 5 s–60 °C 1 min | 1 | |
2 | 95 °C, 5 min | 95 °C, 5 s–60 °C 1 min | 2 | |
3 | 95 °C, 5 min | 95 °C, 5 s–60 °C 1 min | 3 | |
4 | 95 °C, 5 min | 95 °C, 5 s–60 °C 1 min | 4 | |
5 | 95 °C, 5 min | 95 °C, 5 s–60 °C 1 min | 5 |
