Figure 4 | Scientific Reports

Figure 4

From: The programmed DNA elimination and formation of micronuclei in germ line cells of the natural hybridogenetic water frog Pelophylax esculentus

Figure 4

Chromatin in the micronuclei in contrast to gonocytes nuclei is inactivated and accumulates heterochromatin modification. 3D imunofluorescence of whole mount gonads of diploid P. esculentus females at 32 Gosner stage: (a) Chromatin in the micronuclei, but not in the gonocytes nuclei, revealed decreased level (arrow, upper micronucleus) or even absence (arrow, lower micronucleus) of acetylated histone Н4, the modification corresponding to active chromatin. Some micronuclei still retain level of H4Ac similar to main nucleus (arrowhead). (b) Chromatin in the micronuclei accumulates H3K9me3 modification indicating its inactivation and heterochromatinization (arrowhead). Some micronuclei still retain level of H3K9me3 similar to main nucleus (arrow). Images represent single z-section of the gonad. Scale bars 10 µm.

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