Figure 2
R568 anti-secretory effects under cholera toxin-stimulated conditions. Mouse intestines were pretreated with cholera toxin, in vivo, in live animals to induce hypersecretion (see Methods), followed by examining Isc changes of pre-treated intestines, ex vivo, in Ussing chambers. Summarized are steady-state Isc changes of these pretreated intestines in response to the sequential serosal additions of R568 (10 µM) and lidocaine (Lido, 1.6 mM)/TTX (2 µM). The R568 anti-secretory effect is present in colons of wild-type mice and mice expressing neuronal CaSR (villinCre/Casrflox/flox or villin mice) but is absent in mice lacking neuronal CaSR (nestinCre/Casrflox/flox or nestin mice). NS, no significance.
