Figure 3 | Scientific Reports

Figure 3

From: Allergy immunotherapy restores airway epithelial barrier dysfunction through suppressing IL-25 -induced endoplasmic reticulum stress in asthma

Figure 3

Specific-allergen Immunotherapy increases the level of Treg cells and decreases level of IL-25. (a) CD4+ CD25+ Foxp3+ Treg cells in spleen tissue of mice were detected using flow cytometry. (b) The percentage of CD4+ CD25+ Foxp3+ Treg cells of each mice were shown in the scatter plot. 8 × 104 splenocytes were analysed in each sample. (c) The expression of IL-25 in BALF was measured using ELISA. (d) The expression of IL-25 in lung tissue was detected by western blots. (e) Relative changes in the density of IL-25 and β-actin was analysed. Blots in panel (d) have been cropped, and full blots are presented in Supplementary Fig. S3. All data were represented as means ± SEM (n = 6, one-way ANOVA with Tukey’s post hoc, significant differences were defined as p < 0.05).

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