Figure 4 | Scientific Reports

Figure 4

From: Allergy immunotherapy restores airway epithelial barrier dysfunction through suppressing IL-25 -induced endoplasmic reticulum stress in asthma

Figure 4

Specific-allergen Immunotherapy decrease airway epithelial apoptosis and repairs impaired epithelial tight junction in Der f-sensitized mice. (a) Epithelial apoptosis in airway of mice was detected by TUNEL. Nucleus stained by substrate diaminobenzidine (DAB) represent apoptosis occurrence. (original magnification, ×200). (b) The percentage of apoptosis cells in airway was measured at 10 times at random fields in each group. (c,d) Fluorescence intensity of tight junction protein ZO-1 (arrow), E-cadherin was observed by confocal laser scanning microscopy. (e) Expression of Bcl-2, Caspase-3, β-actin in lung tissue were measured by western blot. (f,g) Relative changes in the density of Bcl-2, Caspase-3 and β-actin were analysed. Blots in panel (e) have been cropped, and full blots are presented in Supplementary Fig. S4. All data were represented as means ± SEM (n = 6, one-way ANOVA with Tukey’s post hoc, significant differences were defined as p < 0.05).

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