Figure 3
From: Optimized delivery of siRNA into 3D tumor spheroid cultures in situ
Primary organoid uptake of siRNA is more efficient when complexes are formed with serum. Representative confocal images of VilCreER Apcfl/fl KrasG12D/+ primary mouse organoids showing localization of control-Cy3 siRNA 2, 6 and 24 hours post transfection with siRNA formed in (A) Opti-MEM or (B) 10% serum using RNAiMax. White scale bars indicate 150 μM. Representative confocal images of primary wild type mouse organoids showing localization of control-Cy3 siRNA 2, 6 and 24 hours post transfection with siRNA formed in (C) Opti-MEM or (D) 10% serum using RNAiMax. White scale bar indicates 80 μM except for 24 hours where it indicates 130 μM. White/black arrows indicate the location of the matrigel boundary.
