Figure 6
From: Optimized delivery of siRNA into 3D tumor spheroid cultures in situ
BSA can facilitate matrigel entry of siRNA, but not spheroid uptake. Representative confocal images of SW1463 spheroids showing localization of control-Cy3 siRNA at 2, 6 and 24 hours post transfection with siRNA formed in (A) 10% (B) 1% (C) 0.1% or (D) 0% BSA (no serum present) using RNAiMax. (E) Summary graph showing the mean internal spheroid fluorescence post transfection using siRNA formed with a dose-response of BSA. Data represents mean ± 1 s.d (n = 3).
