Figure 7
From: Optimized delivery of siRNA into 3D tumor spheroid cultures in situ
10 kDa dialyzed serum is also efficient for matrigel and spheroid uptake of siRNA. Representative confocal images of SW1463 spheroids showing localization of control-Cy3 siRNA at 2, 6 and 24 hours post transfection with siRNA formed with (A) 10% (B) 1% (C) 0.1% or (D) 0% dialyzed serum using RNAiMax. (E) Summary graph showing the mean internal spheroid fluorescence post transfection using siRNA formed with a dose-response of dialyzed serum. Data represents mean ± 1 s.d (n = 3). Statistical significance is denoted by ***P < 0.0001 (NS = not significant) as analyzed by a paired student’s t-test.
