Table 1 Overview of raw and processed reads over sequencing time.

Raw data	1 h	2 h	4 h	8 h	16 h	24 h
Total reads	207417	425407	677698	961985	1356358	1422774
Mapped reads (%)	99.08	99.12	99.09	99.05	98.98	98.95
Error rate (%)	12.68	12.57	12.51	12.52	12.66	12.74
Minimal read size (bp)	100	100	100	100	100	100
Maximal read size (bp)	4458	4458	4458	4475	4475	4475
Average read size (bp)	511	509	508	505	501	499
Processed data	1 h	2 h	4 h	8 h	16 h	24 h
Total reads	24874	52139	84685	121185	173529	181544
Mapped reads (%)	99.13	99.09	99.04	99.02	99.00	99.00
Error rate (%)	2.92	2.83	2.84	2.83	2.84	2.85
Minimal read size (bp)	701	701	701	701	701	700
Maximal read size (bp)	2780	2790	2811	2817	2832	2852
Average read size (bp)	995	997	996	995	992	990

This following statistics were collected: read number, percentage of reads mapped to the reference sequences and related error rate, and minimum, maximum and average read size. Raw data: read data after removal of PCR adapters; Processed data: read data after removal of PCR adapters and retaining sequences of 100 bp or longer, error correction and quality trimming (see section “Materials and Methods”).

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