Figure 4

DNA-binding and Protection Assay of PDMAEA. (a1–a4) Assays of DNA-binding potency of PDMAEA during the following period of time: 30 min, 24, 72 and 120 hours. b1–b3) Protection assays of PDMAEA. Comparison of PDMAEA protective role with PEI as standard agent in gene transfer (b1). DNA protection and dissociation pattern after one week of polyplex formation, before and after treated with DNase I respectively (b2 and b3).